Composition and application principle of rat interleukin 4 (IL-4) enzyme-linked immunosorbent assay kit

This kit is for research use only.

Detection range: 48T

3pg/ml-120pg/ml

purpose of usage:

This kit is used to determine the content of interleukin 4 (IL-4) in serum, plasma and related fluid samples of rats.

Experimental principle

The kit uses a double antibody sandwich assay to determine the level of interleukin 4 (IL-4) in the specimen. The microplate was coated with purified rat interleukin 4 (IL-4) antibody to prepare a solid phase antibody, and interleukin 4 (IL-4) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP. The labeled interleukin 4 (IL-4) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with interleukin 4 (IL-4) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the concentration of rat interleukin 4 (IL-4) in the sample was calculated from a standard curve.

Kit composition

Specimen requirements

1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.

2. Samples containing NaN3 could not be detected because NaN3 inhibited horseradish peroxidase (HRP) activity.