The beautiful Qinghai-Tibet Plateau is full of magic and treasures. The Cordyceps sinensis, which grows over 4,500 meters above the plateau and is known as the "Snow Mountain Treasure," is one of them. Cordyceps sinensis is a combination of insects and fungi. It is produced by the growth of larvae of Cordyceps sinensis that leech from the larvae of the lepidoptera bat moth. It is a medicinal and edible treasure and is known as the "King of Medicine." There are more than 300 species of Cordyceps all over the world, but only Cordyceps sinensis produced in the Qinghai-Tibet Plateau has a unique medicinal value. Folks have long had the idea that “winter is insect, summer is grass, and Cordyceps is a treasureâ€. Because of the extremely high medicinal value, the demand at home and abroad is increasing, but the natural Cordyceps resources are limited. The average annual yield in Qinghai Province, the main producing area, is about 30 tons, accounting for more than 60% of the national output, and the annual demand is far away. Far more than 100 tons, so the market price per kilogram of Cordyceps sinensis has risen from 20 yuan before the 1980s to 20,000 yuan now, and the price has increased by as much as 1,000 times in 20 years. It is a rare shortage of medicinal herbs. How to use artificial culture methods to obtain more Cordyceps sinensis for public health services has always been a target for research institutions, scholars, and researchers, and it is also an urgent problem to be solved in the healthcare industry. Isolation of strains of Cordyceps militaris There are two ways to artificially cultivate Cordyceps sinensis: First, artificially feed the host worms and inoculate the host worms with Cordyceps sinensis, making it an artificially infected Cordyceps sinensis. However, this method has never been used due to insect-repellent techniques and inoculation techniques. Although it has been possible to accidentally attach one or two artificial caterpillars from several stem host insects, it is difficult to maintain the insects and the cost is too high to be obtained in recent years. Economic benefits. Second, the isolated Cordyceps sinensis was inoculated into an artificially prepared fluid culture medium, and a large number of Cordyceps sinensis hyphae were propagated through an industrial fermentation technique. Since natural Cordyceps is also used by Cordyceps sinensis to grow hyphae from the body fluid of bat moth larvae, the hyphae occupy all of the body and form Cordyceps sinensis. If this artificial production method can be successfully studied, we can use industrial methods to mass produce cordyceps mycelium to meet the needs of medicine and health products. The key issue is that the strain must be correct. Therefore, how to accurately separate the true Cordyceps sinensis and scientifically prove it is Cordyceps, has become the most important main direction. It is recognized by mycological circles that there are more than 300 species of Cordyceps in the world. To prove that the strain you have obtained is the asexual stage of certain Cordyceps sinensis, it must be able to complete its entire development cycle. That is: 1. Under sterile conditions, the growth and development cycle of mycelium growth sub-spores growing in the container is completed. The "sub-seat" (ie, Cordyceps sinensis) is equivalent to the heading of higher plants. Finally, it forms the capsules in the sub-seats and grows ascospores (which are the seeds of higher plants). The ascospores are the seeds of the sexual stages of the fungus. This artificially cultivated seed (ascospores) is the same as the wild natural cordyceps seed (ascospores). The growth of Cordyceps bacteria sterilized in the container to the later stage, in the mycelium can grow an asexual spore, said "conidia", which is characterized by identification of the bacteria in the classification with the other species of the main basis. Second, the isolated bacteria directly inoculated to the host worm (bat moth larvae) to be able to form the same whole plant Cordyceps militaris. The above two ways can complete one, and it can be confirmed that the strain you isolated is a pure cultured fungus of Cordyceps sinensis, that is, its asexual stage. At present, the strains used by Ruidong Ke Industrial and Trade Co., Ltd. for industrial large-scale production are the first to be separated by researchers of Shen Nanying, and have been verified in the early 1980s and late 1990s, and are also the only ones in China. Both proven methods can complete the correct Cordyceps species throughout the development cycle. The famous China Cordyceps expert Shen Nanying was the first person to discover the asexual stage of Cordyceps sinensis. Shen Lao paid more than forty years of hard work and unimaginable costs for this research topic. In 1964, the researcher Shen Nanying was transferred to juniors and young people for grassland research. During this period, he noticed that many people are digging a medicinal material ---- Cordyceps sinensis, a grassy non-grass, and insect-like non-worm-like thing. What is this? Why is it so great? Curiosity has driven him to germinate. After studying the idea of ​​Cordyceps, he took a long journey to study Cordyceps. Shen Nanying and the research group’s comrades had to reclaim hundreds of thousands of host insects every time they went uphill. Due to lack of oxygen and fatigue, severe altitude sickness caused him to have a splitting headache, chest tightness, and blockage. However, if you want to study Cordyceps, field excavation, field excavation is essential. At times, the volatile weather on the plateau has also caused great trouble for field work. Shen Nanying has survived with a strong will. At that time, some leaders believed that Shen Nanying was a deputy director of the Grassland Research Institute. The main business should be the grassland. He was hooked on Cordyceps. He thought he was not doing a good job, and Shen Nanying did not give up the ideals in his heart, even under pressure. , persistently study hard. There are two methods to separate the strains and cultivate them artificially. This is the tissue separation method. The immature fresh Cordyceps sinensis is collected, cleaned, and the surface is sterilized. The skin is cut off under aseptic conditions to avoid the intestinal tract and the white tissue is cut out. , cut into pieces, and then pick it up from the surface of the culture medium and cultivate it at a suitable temperature. One is the ascospore culture method. Transplant the immature wild natural Cordyceps into a nursery, cover it with a sterilized paper bag, and attach the populated ascospore to a paper bag. The paper bag is dried and used. Purify by centrifugation, obtain purified spores, dilute with sterilized soil leaching solution, inoculate on the isolation medium and incubate at 18°C ​​at room temperature, pick out the germ-like spores that have germinated under the low-temperature microscope, and have no contaminated asbestos around, and move them to a sterile petri dish. In isolation and culture. The key to these two separate culture methods lies in the medium, since Cordyceps is parasitized in the bat moth larvae, which requires that the nutrient content of the medium is similar to the body fluid of the bat moth larvae. In the beginning, it was necessary not only to collect Cordyceps sinensis to isolate strains, but also to excavate the bat moth larvae that had not been parasitized by Cordyceps sinensis to extract sterile body fluid production medium. Thus, the workload was great, in order to obtain more Cordyceps Host insects were used for the first time to isolate the culture medium of Cordyceps sinensis. After obtaining Cordyceps sinensis, they also needed to find a medium that could replace the host insects for easy preparation. Shen Nanying often stayed in the Cordyceps collection area, and tended to separate and cultivate while studying. Formulate medium formulation. Whether it is inappropriate or inappropriate, for Shen Nanying, he is more familiar with failure, but he is more aware that every failure is actually a step closer to success, even if only a little bit. He finally developed a medium formulation that was consistent with the larvae of bat moth larvae. This not only enabled the successful cultivation of Cordyceps fungal culture, but also made it possible to produce hyphae by industrialized fermentation in the future. Fermentation production development and utilization discovered the asexual phase of Cordyceps sinensis--Cerviceri is not the end. As a scientific and technological worker, Shen Nanying thinks more about what method can be used to artificially cultivate cordyceps mycelium industrialization. The scale of production, the technology will be transformed into real productivity, to contribute to society, for humanity, and truly produce a large number of artificial Cordyceps mycelium for public health services. So Shen Nanying started the trek again. He traveled thousands of miles to Hangzhou. He cooperated with Huadong Medicine Group and used the production conditions of the pharmaceutical factory as a pilot practice base to produce a new medicine named “Bailing†capsule with Cordyceps sinensis. Now, it is a brand-name product in Zhejiang Province and has become the brand-name new drug with the largest sales volume, the best drug efficacy, and a wide range of uses. The successful development of the "Bailing" capsule is the first step in the application of the artificial hyphae hyphae, and is the first attempt by Shen Nam-ying to apply his scientific research results to the production of enterprises. It is the first time that the artificial Cordyceps mycelium has served society. leap. Summing up the achievements of scientific research over the years, Shen Nanying's researcher applied for the invention patent of “Chinese Cordyceps sinensis's fermentation production method†in April 1997. The patent was granted in early 2001. This patent enables the industrialized fermentative production of mycelium to be realized, and the production cost is low, the production speed is fast, the fungus is pure and free of bacteria. For larger-scale production of mycelium, a wider range of development and application, Shen Nanying has been looking for more suitable partners. When confronted with Tang and Shan, the entrepreneurs who also share the spirit of “serving public health and revitalizing the motherland’s medicine,†Shen Nanying feels that the dream of many years can finally be realized. The two sides can hit it off. This combination is the combination of Cordyceps sinensis and the company. This combination is the beginning of industrialized mass production of Cordyceps sinensis. This combination is the basis for the further development and utilization of Cordyceps sinensis. This combination is the symbol of Ruidong Company climbing the peak of biotechnology and picking the pearl of Chinese medicine. Ruidong Company takes the biotech road not the castle in the air, and the professional research team with Shen Nanying as the technical director is the cornerstone of development and application of a patent and three technologies of Shen Lao, namely the invention patent of the “fermentative production method of Chinese Cordyceps sinensisâ€. , Cordyceps sinensis artificial separation technology, Cordyceps artificial culture technology and Cordyceps sinensis industrial production technology, in order to achieve the second leap in the application of artificial Cordyceps mycelium development of downstream products, a wider range for the public Health service. By isolating the species from the native Cordyceps militaris, the laboratory optimized and expanded the culture, and three stages of commercial large-scale production in the fermented moth resulted in the Mycelia hirsutum. During the cultivation and fermentation process, the environment and the natural environment are as consistent as possible. Industrially produced mycelium is fermented and enters the tank after hydrolyzation. No culture medium residue is present. The purity of the hyphae is high, and the species is in the middle. The hyphae of the native Cordyceps militaris are equivalent in active ingredients, pharmacological effects and clinical efficacy, in which the polysaccharide and amino acid content is higher than that of natural Cordyceps, and the price is only one-tenth to one-twentieth of that of natural Cordyceps, and thus Only this valuable Chinese medicine of Cordyceps sinensis can enter the homes of ordinary people for consumption by the general public. The natural Cordyceps sinensis used in the prescription of traditional Chinese medicine can be replaced by artificial Cordyceps mycelium. “One patent and three technologies†created a new era of human production of cordyceps mycelium and a milestone in the history of biotechnology development in the world. Ruidong Corporation contributes to public health and contributes to the modernization of traditional Chinese medicine, and is a high-tech enterprise for biotechnology. Make an example. Nowadays, when things are recovering and spring is warm, researchers from Shen Nanying, who are over seventy years old, will lead Ruidong's technicians to go to the Qinghai-Tibet Plateau to isolate strains. This once-year field collection has become a habit for the old experts. Cordyceps sinensis grows on the ground during the first half of May each year. It just grew out of Cordyceps sinensis, its body is hard, and surface bacteria have not penetrated into the body. It is a good time to isolate Cordycepin and it is time to get Cordyceps sinensis authenticity. - This is the case with Chinese hairy spores, and it is not so for development companies.
