The key technology of planting seedlings of sea cucumber

First, the selection of pro-enlistment
1. The choice of pro-ginseng is best to choose the pool of sea cucumber, because it is more synchronous and more plump. First of all, the skin is non-injury, not filthy, followed by the size of the individual between 250 and 350 grams, the gonads account for more than 15% of body weight, body wall accounts for more than 50% of body weight. 400-500 heads of water per 1000 cubic meters of seedlings are needed, and the amount of eggs per female is about 3 to 5 million.
When the pro-ginshen was first caught, it was placed in a long pot, generally in the form of an ellipse or shoe sole in a short period of time. The females accounted for the majority, and if they were long, the males accounted for the majority. Choose elliptic or sole type, this is more in line with nursery requirements.
2. Pro-transportation is generally better with wet transport, and it is transported back at around 4 o'clock in the morning when the temperature is low, the water temperature is low, and the traffic is unobstructed. The specific method: use plastic vats of 50 to 70 liters each, take the original culture tanks filled with seawater, and put a bottle of frozen mineral water that was prepared in advance to be used. Each bottle contains 25 to 35 pro-participants. Within 10 to 12 hours of transportation, there are few pro-species filthy individuals.
Second, the cultivation of parental and promote production
1. When the parental parameter is brought back into the room and stored, the natural water temperature is required, and the salinity is the same as that of the original pool. After it has discharged the excrement, change the pool to water at 5 pm to be produced. The process of collecting and transporting pro-ginseng is a relatively strong stimulus, and most of them can produce a batch of eggs. The eggs produced in this way are of better quality and no one has to breed them. If it is not produced, hold it for 3 to 5 days, change the water once a day, not too much stimulation, until the first 5 days, it will not be produced and it will be combined with the stimulation of the river and the stimulation of the flowing water. Put the pro-ginseng in the empty nursery pond. About 40 minutes, then use a hose to impact the parent for about 20 minutes. This can be spawned 2 to 4 hours after stimulation, spawning more at 9 to 12 o'clock in the evening, should be sent to watch on duty.
2. Disposal measures after spawning (1) Water requirements. The cultivation water is filtered through a sedimentation tank and a sand filter, and then passed through a 1000-mesh filter bag into the pool. Turbidity should be 50 ~ 100 mg / l, just EDTA sodium 3 g / m 3 complex heavy metals, salinity 27 ~ 32, water temperature is generally 17 ~ 20 °C. Put in 2 grams of antibacterial/m3, such as oxytetracycline and streptomycin.
(2) Pro-responsiveness before spawning, usually 8 to 9 o'clock in the evening. The male ginseng was first refined, and about 0.5 to 1 hour later, the ginseng was stimulated by semen to start spawning. At this time, the male ginseng is singled out and placed in another pool for sale. Spawning ponds generally have a water depth of about 0.7 meters. After production is completed, all of the parental ginsengs are moved to another pool for cultivation. The egg pool water will be added to 1.4 meters. At this time, micro-aeration and artificial burping should be performed, and each snoring should be done manually. Once an hour. The role of snoring is to evenly distribute the fertilized eggs and prevent local death of oxygen. This results in the death of the fertilized eggs and the poor quality of hatched larvae, which results in adequate dissolved oxygen during the development of the fertilized eggs.
(3) The next morning, quantify the fertilized eggs. The hatching density should be "small and not large," and it is usually 8 to 10/ml. If the density is too large, it is necessary to divide the pool. The method is to use a siphon tube to suck the pool water into the surrounding pool. In this case, the water body should be stirred up and down to make the egg compartment even.
Third, the cultivation of planktonic larvae
1. Selection of breeding time and method for planktonic larvae should be carried out before development of fertilized egg to spin-off period to small ear-like larvae and digestive tract opening. Selection methods: When larvae develop to be able to swim, strong larvae are mainly distributed in ponds. In the upper and middle layers, first move the oxygenation tube out of the seedling pool; after stopping for 1 hour, observe with a flashlight. When a large number of larvae concentrate on the surface of the water, use a 300-mesh sieve to pull out the larvae floating in the pool water and the upper layer. In the prepared water tank, after being quantified, it is distributed to the prepared nursery ponds, and the total amount of larvae is controlled to be 0.5-1/ml.
2. Daily management (1) Change water. On the first and second days, the method of adding water was used to gradually fill up the water. On the third day, the water was replaced with a 250-mesh cage every day. The amount of water exchanged was 60% to 70% of the total pool. The natural water temperature and salinity remained unchanged. . After each change of water, supplement EDTA sodium salt 3 g/m3. The cages are rinsed with detergent, rinsed with clean water and dried.
(2) Feeding of bait. The sea cucumber larvae cannot digest the planktonic algae that have cell wall. The nursery experiments and production practices show that D. salina, C. capsularis, P. crinae, P. tricornutum are mixed and used with marine red yeast, and the mixed feeding of larvae with dry yeast tablets for aquatic production can also achieve better nursery effects.
The quality requirements of the monocytic algae: First, the algae algae fluid has this unique bright color, microscopic examination without protozoa, no aging cells. Occasionally found protozoa can also be fed, but cloudy, rain, lack of light, protozoa often proliferate, in the nursery must pay attention to timely microscopy. Second, the culture density of single-cell algae is: 1 million cells/ml of Dunaliella salina, 2×10 2 cells/ml of Phaeodactylum tricornutum, C. trichocarpa, and Chaetoceros.
Monosodium algae and other bait feeding methods. When the echinian larvae of the small ear larvae open, they are opened with a small amount of Dunaliella salina and marine red yeast. The density of ear-like larvae is 0.8 to 1/ml, the daily feeding amount is 3000 cells/ml, the salt algae account for 50%, chaetoceros and 50% of genus genus: moon ear larvae at 5000 cells/ml, Chaetoceros, Dunaliella, and C. sulphurae each occupy 1/3; big ear larvae at 8000 cells/ml, Chaetoceros, Dunaliella Each accounted for 40%, and the new moonroots was 20%. After 3 feedings, once every 8 hours, feeding after changing water, no feeding until the larvae develop into the larva-shaped larvae stage, basically no feeding, and less investment when there are large-eared larvae that do not change into larvae. Other baits, such as marine red yeast, and yeast tablets for aquatic use, can be alternately cast or single-shot at 2 g/m3 daily, and milk powder can also be used, generally 0.1 g/m3 per day.
3. Microscopic examination and observation After feeding for half an hour after microscopic examination, if most of the larvae have obvious color in the stomach food, and see the bait particles, then the amount of bait is appropriate; if the bait is still sufficient in the stomach after feeding for half an hour, However, the next time the larvae are empty, the part of the larvae before the feeding will be insufficient, and the feeding amount should be increased. If the larvae in the stomach before the feeding are still rich in food and the intestines are filled with feces, the feeding amount is too large.
After a few larval development stages, Stichopus japonicus can develop into juvenile ginseng, and it takes about 11 to 13 days to undergo regular microscopy. At least once per pool, it is necessary to understand the development of feeding and development. The length of small ears is 450 to 600 microns. Ears of 600 to 800 microns, ears of 800 to 1000 microns, and an average daily increase of 50 microns are normal. The development of ear larvae mainly depends on the development of water cavity. The water cavities of the small ear larvae are round cystic. The water cavities of the middle ear larvae are long cystic. The water cavities of the big ear larvae appear concave and convex peanuts on a long saccate basis. The larvae of the large-eared larvae form fingers in the later stage. The larval arm shows five pairs of balls, with five tentacle primordium and irradiating water tube primordium. The larval larvae grow into large ears. Half of the larvae developed into juvenile saplings from 1 tentative larva of 1 to 2 days into the juveniles.

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        Organic Clarified Goji Berry Juice


Appearance

1) Color: Brownish red or deep brownish red 
2) Taste & Odor: Fresh Goji Berry juice taste and smell after sterilization, no peculiar smell.
3) Histomorphology: The product is transparent, allowing micro precipitation after standing 
4) Impurity: No visible foreign impurities 


Physics & Chemical Specification

1) Brix(%): ≥13.0 

2) Total Acid(g/100mLas Citric Acid): ≥0.5 
3) pH: 3.5-4.1 
4) Light Transmittance(620nm, %): ≥10 


Microorganism  Specification

1) TPC (CFU/mL): ≤100 
2) Yeast (CFU/mL): ≤20 
3) Mould (CFU/mL): ≤20 
4) Coliform (CFU/mL): ≤1 


Additive

NO


Country of Origin

ZHONGNING, NINGXIA, CHINA


Package

1) Inner package is 200kgs aseptic bag, outer package is opening steel drum.
2) Inner package is 25kgs aseptic bag, outer package is opening steel drum.


Storage

Should be stored at room temperature in clean, cool, dry warehouse, prevent sun, rain, and not be stored with corrosive, toxic, and smelly item. With these conditions, product shelf life is 12 months. (suggestion: 5-8℃).  

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