ELISA test kit guide | ||
| step | common problem | solution |
| Experimental preparation | 1 The sampler is inaccurate; especially the sampler below 10 ul has a great impact on the results; 2 poor thermal insulation equipment; 3 Washing water is not standardized. | 1 calibration sampler; the following 10 ul sampler is best to use imported products (Finland, France, etc.); 2 Carefully adjust the temperature to 37 ° C, the water bath is better; 3 Deionized water or distilled water must be used, and tap water, mineral water, etc. must not be used. 4 Read the instruction manual carefully. |
| Sample loading | 1 The sample is not accurate; 2 No mixing. | 1 Be careful when loading the sample. After loading, check again to prevent leakage and mis-addition. 2 After the sample is added, the mixture is repeatedly sucked 3 times to prevent the serum from collecting at the bottom of the well, resulting in non-specific adsorption. |
| washing | Incomplete washing | 1 Each time the water is washed, it should be still for 30 seconds; 2 Use absorbent paper as the liner, and buckle the moisture in the dry hole after each washing; 3 Plastic washing bottles are available. |
| Enzyme reaction | Missing | After the drop, carefully check each hole |
| Result judgment | 1 Including negative control wells, the color development of each well is generally deep; 2 There is no coloration in each well including the positive control; 3 The positive control did not develop color, while the other samples showed normal color; 4 Some specimens are not dark in color and it is difficult to judge positive. | 1 may be insufficient washing; excessive loading; excessive incubation time is too high; follow the instructions to re-operate. If there is no improvement, you can contact the manufacturer; 2 It may be that the sample or enzyme solution is missing; the incubation time is too short and the temperature is too low; the activity of improper storage of the reagent is decreased; follow the instructions. If there is no improvement, you can contact the manufacturer; 3 Positive control missed, added or failed. Re-operate. If there is no improvement, you can contact the manufacturer and ask for the reference product; 4 Re-operate. It is best to use a microplate reader to measure the light absorbance and judge according to the P/N value standard. |
| Reagent preservation | Not standardized | Always store at 2-8 °C. Do not be too high or frozen (the enzyme solution will fail after cryopreservation). |
| Reference substance | The lyophilized product | According to the control tube, add the corresponding volume of deionized water or distilled water to reconstitute, fully dissolve and mix. Can be used according to the instructions. |
| other | 1 If you have any comments or suggestions on the reagents, please feel free to contact us by phone, email, internet, etc. We will definitely provide a warm service. Thanks a lot! 2 The above mentioned equipment, our company also has supply, if necessary, welcome to consult Jingmei Biological to order. | |
Spice Novelties Co.,Limited , https://www.wholesale-adult-toys.cn